LabCoat Live Courses
Enhance your understanding of basic molecular biology techniques with hands-on, classroom and online basic trainings.
Courses Offered at Training Centers
MB-101: Molecular Biology Basics for PCR Applications (2 days)
$1750 (with 12 P.A.C.E.® contact hours)
Who Should Attend?
This basic, introductory-level course is designed for people with little or no experience in a molecular biology laboratory or for those wishing to refresh past experience. Attendees will benefit from having a combination of classroom training and hands-on experience in the laboratory, as well as from interacting closely with fellow attendees and application scientists over a 2-day period.
This course covers basic theoretical concepts and laboratory techniques of molecular biology, as they apply to PCR-based applications such as pharmacogenomics, gene expression analysis, genotyping, and DNA sequencing. Lecture topics include nucleic acid structure and function, DNA replication, nucleic acid purification, design and theory of the polymerase chain reaction (PCR), and real-time PCR theory and fundamentals of data analysis. Laboratory exercises include DNA purification and quantification, PCR, analysis of DNA by gel electrophoresis, and real-time PCR assays and analysis.
At the conclusion of this training, participants should be able to:
• Explain and describe basic features of DNA and how it functions in a eukaryotic cell.
• Determine an appropriate nucleic acid preparation technique for different starting sample types.
• Interpret fragment size and quantity in DNA samples by gel electrophoresis and other analysis methods.
• Recognize the difference between PCR and real-time PCR and know when to apply each of them.
• Apply course concepts to design basic experiments using PCR amplification.
The Thermo Fisher Scientific Training Center is an approved provider of continuing education programs in the clinical laboratory sciences by The American Society for Clinical Laboratory Science (ASCLS) Professional Acknowledgment for Continuing Education (P.A.C.E.®) program.
Assessment criteria: class participation and quiz.
Participants successfully passing the quiz (80%) will be awarded 12 contact hours.
Tuition includes all course materials, laboratory reagents, and consumables. Light breakfast, snacks and lunch are also provided. Travel, accommodation, and other incidental expenses relating to the training are the responsibility of the attendee.
Technology required: none from class partipant. During the class, particpant will have access to laptops and laboratory equipments.
For more information about our course schedule, please contact us at firstname.lastname@example.org.
MB-205: Introduction to CRISPR-Cas9 Genome Editing - Design to Analysis (4 days)
Recent discoveries have dramatically changed our ability to precisely edit genomic DNA. Tools like TAL effector nucleases and CRISPR systems have enabled researchers to rapidly, accurately and reliably generate double-strand DNA breaks, and these high efficiency gene editing systems provide a basis for innovative applications in cell engineering.
For those interested in utilizing CRISPR-Cas9 genome editing to accelerate their discovery, getting started in this rapidly changing field can be daunting. This course will walk you through a basic CRISPR cell engineering workflow, from design and synthesis of target-specific guide RNAs to analysis of gene editing efficiency. During the 4-day workshop of introductory lectures, discussions, and hands-on laboratory work, our experienced trainers will guide you through knockout and knock-in experiments using CRISPR-Cas9 in mammalian cells. Low student-to-trainer ratios in the class will help to ensure you can receive the personal attention you need to successfully learn these new methods.
Who Should Attend?
This is an intermediate-level course with regard to laboratory skills and assumes that attendees will have prior experience in basic molecular biology laboratory techniques, including PCR, and basic mammalian cell culturing techniques.
However, it is an entry-level course for the CRISPR workflow, designed for life science professionals with little or no previous experience in genome editing mechanisms and applications.
Some of these prerequisite requirements can be fulfilled by attending our Molecular Biology Basics for PCR Applications or Cell Culture Basics courses. Please inquire about discounted pricing to attend multiple classes.
Classroom lectures and discussions will include:
Hands-on laboratory sessions will include:
You will receive a detailed course guide containing all lecture materials, laboratory protocols, troubleshooting tips and more to help you get started right away when you return to your lab.
Registrations are limited to 8 attendees per class to ensure a rich educational experience.
Please note that this entry-level course will have very limited information on more advanced topics like multiplexing, screening, clonal isolation, validation processes, and use of variant forms of Cas9 protein. Please let us know of your interest in these topics for potential development of new courses in the future.
Tuition includes all course materials, laboratory reagents, and consumables. Light breakfast, snacks and lunch are also provided each day. Travel, accommodation, and other incidental expenses relating to the training are the responsibility of the attendee.
MB-050: Lab Basics Video Series
Who Should Attend?
This course is designed for anyone new to molecular biology or seeking refresher training. It covers very basic laboratory protocol and chemistry concepts that are frequently used in molecular biology applications.
This course is also a prerequisite to MB-101: Molecular Biology Basics for PCR Applications (2 days).
See below for brief descriptions of each video. We recommend viewing the series in the indicated order.
For Research Use Only. Not for use in diagnostic procedures.