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Life Technologies Introduces New Real-Time PCR System and Associated Training

ViiA 7

July 15, 2010 - Life Technologies Corporation recently announced the expansion of its high quality family of real-time PCR systems with the introduction of a new high-productivity instrument. The Applied Biosystems ViiA™ 7 Real-Time PCR System seamlessly integrates a variety of quantitative PCR (qPCR) and genotyping applications, enabling researchers to enter a new era of lab productivity. The system offers a number of performance and usability enhancements that are expected to advance the understanding of molecular biology in the pharmaceutical discovery and clinical research markets.

Real-time PCR is a common laboratory method that measures PCR amplification as it occurs, cycle-by-cycle, allowing quantitative measurements to be made in the highly reproducible exponential phase of PCR. This enables extremely accurate and precise quantification of nucleic acids over a large dynamic range compared to traditional techniques. Since the introduction of the first real-time PCR instrument by Applied Biosystems 14 years ago, researchers have increasingly relied on applications of real-time PCR to gain a better understanding of the molecular basis of disease.

The ViiA™ 7 Real-Time PCR System has been designed to improve researcher productivity for research that includes how gene expression changes in response to pharmacological agents, and how genetic variation influences response to treatments for disease. The system optimizes and simplifies a host of real-time PCR applications; all integrated and easily accessible from a touch screen. A patent-pending OptiFlex™ System within the ViiA™ 7 System provides the sensitivity to detect a single copy of starting genetic material and offers enhanced multiplexing flexibility.

"In the past decade, quantitative real-time PCR has become a cornerstone technology for the life science researcher, allowing scientists around the world to identify molecular markers for disease and precisely measure how changes in activity at the genome level translate to significant molecular outcomes in living systems," said Peter Dansky, President of Molecular Biology Systems for Life Technologies. "By integrating a broad portfolio of real-time PCR applications on a single high-performance platform, the ViiA™ 7 Real-Time PCR System will vastly increase the productivity of researchers and inspire them to develop novel workflows that lead to an even greater understanding of the molecular basis of health and disease."

The ViiA™ 7 System's software integrates standard real-time PCR applications like nucleic acid quantification, detection of single nucleotide polymorphisms (SNPs), and copy number variation detection with others that include high-resolution melting, protein analysis with proximity ligation technology, and gene expression analysis performed directly from cultured cells without RNA purification.

The ViiA™ 7 System also includes usability enhancements that enable efficient and cost-effective research. Through one-touch protocols, researchers can operate the system without a separate computer, and monitor runs from remote locations. Automated sample loading enables uninterrupted system operation and eliminates the need for robotic pipetting equipment.

The ViiA™ 7 System began shipping in June. We're proud to announce that the following training options are now available:

Basic Principles of Real-Time Quantitative PCR and Instrument Maintenance for the ViiA™ 7 Real-Time PCR System (2 days at Applied Biosystems)
This course is designed for new users who want to gain an understanding of real-time PCR applications and data analysis on the ViiA™ 7 System. Attendees will benefit from having training in a classroom environment, attending hands-on lab practicals, and interacting with fellow attendees and Applied Biosystems application scientists during lecture and discussion sessions. The course is designed to teach (1) proper experimental technique, (2) proper experimental design, (3) software use and data analysis for quantification of standard curve experiments, and (4) proper instrument use, calibration, and maintenance.

ViiA™ 7 Real-Time PCR System Consulting (hourly)
This service is designed for customers who require consultancy for their research projects. Customers will be able to take advantage of the extensive expertise of Applied Biosystems application scientists as they develop and deliver fully customized applications support based on the customer’s unique requirements. Activities include but are not limited to: workflow analysis, technology adoption, laboratory setup, application troubleshooting and optimization, data analysis, specialized applications, and experimental design.

For more information on ViiA™ 7 Real-Time PCR System training, please visit or contact your local Applied Biosystems sales representative.