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Introduction to CRISPR-Cas9 Genome Editing - Design to Analysis (4 days)

P/N: A33133

Course Description

Recent discoveries have dramatically changed our ability to precisely edit genomic DNA. Tools like TAL effector nucleases and CRISPR systems have enabled researchers to rapidly, accurately and reliably generate double-strand DNA breaks, and these high efficiency gene editing systems provide a basis for innovative applications in cell engineering.

For those interested in utilizing CRISPR-Cas9 genome editing to accelerate their discovery, getting started in this rapidly changing field can be daunting.  This course will walk you through a basic CRISPR cell engineering workflow, from design and synthesis of target-specific guide RNAs to analysis of gene editing efficiency.  During the 4-day workshop of introductory lectures, discussions, and hands-on laboratory work, our experienced trainers will guide you through knockout and knock-in experiments using CRISPR-Cas9. Low student-to-trainer ratios in the class will help to ensure you can receive the personal attention you need to successfully learn these new methods.

Course Content

Lectures will include an introduction to Genome Editing and CRISPR-Cas9 technology, and reviews of how to design and generate target-specific guide RNA (gRNA) molecules, different Cas9 and gRNA formats and transfection delivery options, methods for initial evaluation of gene editing efficiency, and high-level overview discussions of experimental design and additional validation steps for the most common applications.

Laboratory sessions will include  gRNA synthesis using PCR and in vitro transcription, delivery of  gRNAs and Cas9 mRNA or Cas9 Protein into a human cell line using lipid transfection and electroporation, and initial screening of cells for editing success by PCR and phenotype analysis.

Registrations are limited to 6 attendees per class to ensure a rich educational experience.

Who Should Attend?

This is an entry-level course with regard to the CRISPR workflow, designed for life science professionals with little or no background experience in genome editing mechanisms and applications.

However, it is an intermediate-level course with regard to laboratory skills and assumes that attendees will have prior experience in basic molecular biology laboratory techniques, including PCR, and basic mammalian cell culturing techniques.

Some of these prerequisite requirements could be fulfilled by attending our Cell Culture Basics course. Please inquire about discounted pricing to attend multiple classes.

Course Content:

Classroom lectures and discussions will include:

  • Review of principles and application of the CRISPR-Cas9 system for genome editing
  • Precise design and synthesis of optimal, pure target-specific guide RNA (gRNA) molecules
  • Comparison of different CRISPR-Cas9 molecular formats (plasmid, RNA, protein) with transfection delivery options and optimization
  • Review of CRISPR experimental design strategies for successful gene editing projects
  • Overview of methods to evaluate and validate gene editing efficiency
  • Workflow case study exercises, with opportunity to receive direct guidance from the trainers in designing your own first genome editing experiment.

Hands-on laboratory sessions will include: 

  • gRNA synthesis using PCR and in vitro transcription (IVT)
  • Generation of gene knockouts (via repair insertion/deletion) in a human cell line by delivering IVT gRNAs and either Cas9 mRNA or Cas9 protein into the cell line using transfection reagents and electroporation.
  • Introduction of a SNP change in a fluorescent protein through homology-directed repair.
  • Determination of insertion/deletion editing efficiency with a PCR-based DNA mismatch method (Genomic Cleavage Detection Assay) and detection of the SNP change through analysis of fluorescent protein expression.                                                                                                                               

Attendees will benefit from detailed guidance by our experienced Application Scientist trainers and interaction with fellow attendees during the hands-on lab sessions, lectures and discussion sessions.

You will receive a detailed course guide containing all lecture materials, laboratory protocols, troubleshooting tips and more to help you get started right away when you return to your lab. Registrations are limited to 6 attendees per class to ensure a rich educational experience.

Please note that this entry-level course will have very limited information on more advanced topics like multiplexing, screening, clonal isolation, validation processes, and use of variant forms of Cas9 protein. Please let us know of your interest in these topics for potential development of new courses in the future.

Other Details:

Tuition includes all course materials, laboratory reagents, and consumables.  Snacks and lunch are also provided each day. Travel, accommodation, and other incidental expenses relating to the training are the responsibility of the attendee.

The price quoted on the website is in the local currency for the location where the training will take place. Our terms and conditions state that “All training prices indicated will vary between countries and currency and are quoted as a guide only. We have to invoice in the local currency of the customer (billing address). We will create a quotation for you upon request. 

Cancellation policy: Thermo Fisher Scientific reserves the right to cancel or reschedule a course up to 10 working days prior to the listed course start date. For more information, please refer to the Terms and Conditions below.

Terms and Conditions

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City/Country   Regional City/Country   Tours & Workshops WEB   Web-Based Training
Start date End date Start time End time Price Language Location
February 26, 2019 March 1, 2019 9:00am 2:30pm £1,666.00 English
April 9, 2019 April 12, 2019 9:00am 2:30pm €2,025.00 English
May 14, 2019 May 17, 2019 9:00am 2:30pm £1,666.00 English
June 25, 2019 June 28, 2019 9:00am 2:30pm €2,025.00 English