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Introduction to CRISPR-Cas9 Genome Editing - Design to Analysis (4 days)

P/N: A33133

COVID-19 Update:  We are actively monitoring the situation with COVID-19. For any courses rescheduled due to travel restrictions we are happy to transfer your registration to a future course date. Please contact with any questions.

Tools like CRISPR-Cas9 have dramatically changed our ability to precisely edit DNA sequence, enabling exciting and innovative new applications in cell engineering. This course will walk you through basic CRISPR DNA editing workflows in mammalian cells, from design and synthesis of target-specific guide RNAs to analysis of gene editing efficiency and sequence results.

Who Should Attend?

This is an intermediate-level laboratory skills course. Although it is an introduction to CRISPR-Cas9 and does not require any background knowledge of genome editing mechanisms or the CRISPR workflow, it is designed for life science professionals who have prior experience in basic molecular biology laboratory techniques, including PCR, and basic mammalian cell culturing techniques.

Some prerequisite requirements could be fulfilled by attending our Molecular Biology Basics for PCR Applications or Cell Culture Basics course(s).

Also note this course is focused on mammalian cell applications, and there will be limited information on more advanced topics like screening and Cas9 variants. The concepts are widely applicable, but our ability to specifically support researchers working with other species or applications is limited. 

Course Content:

Classroom lectures and discussions include:

  • Overview of the CRISPR-Cas9 workflow and common applications
  • Precise design and synthesis of optimal target-specific guide RNA (gRNA) molecules and homology directed repair (HDR) templates
  • Comparison of different CRISPR-Cas9 molecular formats (protein, RNA, plasmid), with transfection delivery options and optimization
  • Overview of methods to evaluate and validate gene editing efficiency and sequence results
  • Detailed review of experimental design strategies, with case study exercises and opportunities to receive direct guidance from the instructors in planning your own genome editing experiment

Hands-on laboratory sessions include: 

  • Generation of gene knockouts in a human cell line by delivering gRNAs and Cas9 Protein or mRNA into the cell line using transfection reagents and electroporation
  • Knock-in of a targeted SNP change in a fluorescent protein through homology-directed repair
  • Determination of insertion/deletion editing efficiency with a PCR-based DNA mismatch method (Genomic Cleavage Detection Assay) and detection of the SNP change through analysis of fluorescent protein expression.                                                                                                                     

You will receive a detailed course guide containing all lecture materials, laboratory protocols, troubleshooting tips and more to help you get started right away when you return to your lab.

Registrations are limited to 6 attendees per class to ensure low student-instructor ratios and a rich educational experience.

Other Details:

Tuition includes all course materials, laboratory reagents, and consumables.  Snacks and lunch are also provided each day. Travel, accommodation, and other incidental expenses relating to the training are the responsibility of the attendee.

The price quoted on the website is in local currency for the location where the training will take place. Training prices will vary between countries and currency and are quoted as a guide only. Invoices will be completed in the local currency for each customer based on location. A quotation can be raised upon request. Please refer to the Terms and Conditions section below for more information

Cancellation policy: Thermo Fisher Scientific reserves the right to cancel or reschedule a course up to 10 working days prior to the listed course start date. For more information, please refer to the Terms and Conditions below.

Terms and Conditions

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