Cell Culture Basics – Optimizing Lab Techniques (3 Days)
Mammalian cell culture is an integral tool in many workflows of biological study, and this course is designed to provide key tips and best practices for maintaining healthy cell lines and obtaining the most accurate and reproducible results in downstream applications.
Who Should Attend?
This basic-level course is designed for life science professionals or students with little or no direct experience working in a tissue culture laboratory, or those wishing to refresh or expand on past experience.
Some background knowledge of biology and high-level familiarity with structure and functions of cells is expected.
Course Content and Objectives:
Lectures include reviews of basic cell culture concepts and terminology, biosafety and usage of key equipment for optimal sterile technique, growth characteristics of typical mammalian continuous cell lines, important considerations for successful cryopreservation, overview of transfection technologies, methods to confirm cell identity and health status, and brief introduction to concepts in more advanced primary and stem cell culture.
Laboratory sessions include cell counting and passaging of both adherent and suspension mammalian cell lines, cryopreservation (freezing, thawing and viability monitoring), lipid transfection and electroporation, and cell health assays.
At the conclusion of this training, participants should be able to:
Tuesday 8:30am - 6:00pm (Extra time on the first day to maximize the lab experience.)
Wednesday 9:00am - 5:00pm
Thursday 9:00am - 4:30pm
Tuition includes all course materials, laboratory reagents, and consumables. Snacks and lunch are also provided each day. Travel, accommodation, and other incidental expenses relating to the training are the responsibility of the attendee.
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