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LabCoat Live: Cell Line Authentication using Capillary Electrophoresis

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P/N: TRN00132

Price: $300

 

Who Should Attend?

Laboratory personnel interested in learning about the human cell line authentication application using STR DNA profiling and capillary electrophoresis platforms.    

Course Description:

Learn the fundamentals of how to authenticate human cell lines when using STR DNA profiling and capillary electrophoresis systems. This course provides professional, interactive education via online lecture(s) and a self-paced hands-on lab experiment using provided protocol(s) and reagents.

At the conclusion of this training, participants should be able to:

  • Describe the importance of human cell line authentication
  • Explain basic theory of STR DNA profiling
  • Outline the sample preparation workflow and identify key experimental parameters for microsatellite amplification and sizing
  • Analyze fsa. files to identify cell lines

Tuition includes:

  • Three 1-hour interactive online lectures
  • 1 month of access to class webpage, including downloadable notes and protocols, support videos, and question and answer forum
  • A training kit* that includes reagents needed to perform course protocols

* Only 1 training kit will be provided per customer site based upon shipping address as the kit contains multiple reactions

Prerequisites:

  • Functional knowledge of cell culture and molecular biology techniques
  • Functional knowledge of capillary electrophoresis and primary and secondary fragment analysis with GeneMapper Software

Equipment Needed:

  • Access to Applied Biosystems Genetic Analyzer instrument and accessories (3500, 3730 or SeqStudio)
  • A computer with internet access and GeneMapper software
  • Basic laboratory equipment (i.e. pipettes, thermal cycler, bench top centrifuge, mini centrifuge etc.)
  • Kits or reagents for genetic analyzer general operation and calibration to run fragment analysis experiments (capillary array, anode and cathode buffer, POP7 polymer, DS-33 Matrix Standard Kit, MicroAmp 96-well reaction plates, 96-well plate septa, etc.) 


Reserve a spot in one of these courses at thermofisher.com/labcoatlive.  Please email us at labcoatlive@thermofisher.com with any questions.

For Research Use Only. Not for use in diagnostic procedures.

 

Tell a colleague about this course. Copy the following URL:
https://learn.thermofisher.com/courses/view/id/419

CBD   Carlsbad, CA   FRD   Frederick, MD   REG   Regional   WEB   Web-Based Training