LabCoat Live Courses
MB-205: Introduction to CRISPR-Cas9 Genome Editing - Design to Analysis (4 days)
Recent discoveries have dramatically changed our ability to precisely edit genomic DNA. Tools like TAL effector nucleases and CRISPR systems have enabled researchers to rapidly, accurately and reliably generate double-strand DNA breaks, and these high efficiency gene editing systems provide a basis for innovative applications in cell engineering.
For those interested in utilizing CRISPR-Cas9 genome editing to accelerate their discovery, getting started in this rapidly changing field can be daunting. This course will walk you through a basic CRISPR cell engineering workflow, from design and synthesis of target-specific guide RNAs to analysis of gene editing efficiency. During the 4-day workshop of introductory lectures, discussions, and hands-on laboratory work, our experienced trainers will guide you through knockout and knock-in experiments using CRISPR-Cas9 in mammalian cells. Low student-to-trainer ratios in the class will help to ensure you can receive the personal attention you need to successfully learn these new methods.
Who Should Attend?
This is an intermediate-level course with regard to laboratory skills and assumes that attendees will have prior experience in basic molecular biology laboratory techniques, including PCR, and basic mammalian cell culturing techniques.
However, it is an entry-level course for the CRISPR workflow, designed for life science professionals with little or no previous experience in genome editing mechanisms and applications.
Some of these prerequisite requirements can be fulfilled by attending our Molecular Biology Basics for PCR Applications or Cell Culture Basics courses. Please inquire about discounted pricing to attend multiple classes.
Classroom lectures and discussions will include:
Hands-on laboratory sessions will include:
You will receive a detailed course guide containing all lecture materials, laboratory protocols, troubleshooting tips and more to help you get started right away when you return to your lab.
Registrations are limited to 8 attendees per class to ensure a rich educational experience.
Please note that this entry-level course will have very limited information on more advanced topics like multiplexing, screening, clonal isolation, validation processes, and use of variant forms of Cas9 protein. Please let us know of your interest in these topics for potential development of new courses in the future.
Tuition includes all course materials, laboratory reagents, and consumables. Light breakfast, snacks and lunch are also provided each day. Travel, accommodation, and other incidental expenses relating to the training are the responsibility of the attendee.
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