MB-205: Introduction to CRISPR-Cas9 Genome Editing - Design to Analysis (4 days)
Tools like CRISPR-Cas9 have dramatically changed our ability to precisely edit DNA sequence, enabling exciting and innovative new applications in cell engineering. This course will walk you through basic CRISPR DNA editing workflows in mammalian cells, from design and synthesis of target-specific guide RNAs to analysis of gene editing efficiency and sequence results.
Who Should Attend?
This is an intermediate-level laboratory skills course. Although it is an introduction to CRISPR-Cas9 and does not require any background knowledge of genome editing mechanisms or the CRISPR workflow, it is designed for life science professionals who have prior experience in basic molecular biology laboratory techniques, including PCR, and basic mammalian cell culturing techniques.
Also note this course is focused on mammalian cell applications, and there will be limited information on more advanced topics like screening and Cas9 variants. The concepts are widely applicable, but our ability to specifically support researchers working with other species or applications is limited.
Classroom lectures and discussions include:
Hands-on laboratory sessions include:
You will receive a detailed course guide containing all lecture materials, laboratory protocols, troubleshooting tips and more to help you get started right away when you return to your lab.
Registrations are limited to 6 to 8 attendees per class to ensure low student-instructor ratios and a rich educational experience.
Tuition includes all course materials, laboratory reagents, and consumables. Light breakfast, snacks and lunch are also provided each day. Travel, accommodation, and other incidental expenses relating to the training are the responsibility of the attendee.
Please also review our general course policies.
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