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MB-205: Introduction to CRISPR-Cas9 Genome Editing - Design to Analysis (4 days)

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P/N: A33133

 

Recent discoveries have dramatically changed our ability to precisely edit genomic DNA. Tools like TAL effector nucleases and CRISPR systems have enabled researchers to rapidly, accurately and reliably generate double-strand DNA breaks, and these high efficiency gene editing systems provide a basis for innovative applications in cell engineering.

For those interested in utilizing CRISPR-Cas9 genome editing to accelerate their discovery, getting started in this rapidly changing field can be daunting.  This course will walk you through a basic CRISPR cell engineering workflow, from design and synthesis of target-specific guide RNAs to analysis of gene editing efficiency.  During the 4-day workshop of introductory lectures, discussions, and hands-on laboratory work, our experienced trainers will guide you through knockout and knock-in experiments using CRISPR-Cas9 in mammalian cells. Low student-to-trainer ratios in the class will help to ensure you can receive the personal attention you need to successfully learn these new methods.

Who Should Attend?

This is an entry-level course with regard to the CRISPR workflow, designed for life science professionals with little or no background experience in genome editing mechanisms and applications.

However, it is an intermediate-level course with regard to laboratory skills and assumes that attendees will have prior experience in basic molecular biology laboratory techniques, including PCR, and basic mammalian cell culturing techniques.

Some of these prerequisite requirements can be fulfilled by attending our Molecular Biology Basics for PCR Applications or Cell Culture Basics courses. Please inquire about discounted pricing to attend multiple classes.

Course Content

Classroom lectures and discussions will include:

  • Review of principles and application of the CRISPR-Cas9 system for genome editing
  • Precise design and synthesis of optimal, pure target-specific guide RNA (gRNA) molecules
  • Comparison of different CRISPR-Cas9 molecular formats (plasmid, RNA, protein) with transfection delivery options and optimization
  • Review of CRISPR experimental design strategies for successful gene editing projects
  • Overview of methods to evaluate and validate gene editing efficiency
  • Workflow case study exercises, with opportunity to receive direct guidance from the trainers in designing your own first genome editing experiment.

Hands-on laboratory sessions will include: 

  • gRNA synthesis using PCR and in vitro transcription (IVT)
  • Generation of gene knockouts (via repair insertion/deletion) in a human cell line by delivering IVT gRNAs and either Cas9 mRNA or Cas9 protein into the cell line using transfection reagents and electroporation.
  • Introduction of a SNP change in a fluorescent protein through homology-directed repair.
  • Determination of insertion/deletion editing efficiency with a PCR-based DNA mismatch method (Genomic Cleavage Detection Assay) and detection of the SNP change through analysis of fluorescent protein expression.

You will receive a detailed course guide containing all lecture materials, laboratory protocols, troubleshooting tips and more to help you get started right away when you return to your lab.

Registrations are limited to 8 attendees per class to ensure a rich educational experience.

Please note that this entry-level course will have very limited information on more advanced topics like multiplexing, screening, clonal isolation, validation processes, and use of variant forms of Cas9 protein. Please let us know of your interest in these topics for potential development of new courses in the future.

Other Details:

Tuition includes all course materials, laboratory reagents, and consumables. Light breakfast, snacks and lunch are also provided each day. Travel, accommodation, and other incidental expenses relating to the training are the responsibility of the attendee.

For questions or more information, please contact us at customertraining@thermofisher.com.

 

To see class dates for our Customer Experience Centers in Europe, please click here.

 

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CBD   Carlsbad, CA   FRD   Frederick, MD   REG   Regional   WEB   Web-Based Training
Start date End date Start time End time Hours Price Location
August 22, 2017 August 25, 2017 8:30am PT 5:30pm PT   Class is full.
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September 19, 2017 September 22, 2017 8:30am PT 5:30pm PT   Class is full.
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October 24, 2017 October 27, 2017 8:30am PT 5:30pm PT Contact for Price
November 7, 2017 November 10, 2017 8:30am PT 5:30pm PT $2,995.00
December 5, 2017 December 8, 2017 8:30am PT 5:30pm PT $2,995.00