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LabCoat Live: Introduction to qPCR and Absolute Quantification

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P/N: A29801

Price: $300

Who Should Attend?

This course is designed for anyone interested in learning more about the basics of qPCR and the Absolute Quantification (AQ) method of analysis. The course consists of two interactive online lectures, an class website with step-by-step experiment setup guides, a dedicated web discussion board to get questions answered quickly, and a self-paced lab experiment designed to be performed using the provided reagents and protocol.


Participants must have access to an Applied Biosystems™ Real-Time PCR Instrument (7900HT Fast, QuantStudio™ series, 7500 series, StepOne™/StepOnePlus™, or ViiA™ 7 Real-Time PCR System), as well as materials necessary to set up a qPCR experiment (optical plates and adhesive covers, aerosol-resistant filter tips, pipettes, gloves, racks, vortex, microfuge tubes). In addition, participants will need a computer with Internet access and minimum system requirements.

This class assumes attendees have prior knowledge of basic molecular biology concepts, including endpoint (conventional) PCR.

Course Description:

Participants can expect to learn:

  • Basic qPCR theory
  • How to perform an absolute quantitation experiment using a standard curve
  • The experimental parameters for performing absolute quantitation
  • How to analyze standard curve data using Applied Biosystems™ software

Price includes two 1.5-hour interactive online lectures, 1 month of access to class webpage (including downloadable notes and protocol, support videos, question and answer forum), and starter kit (includes reagents needed to perform the course’s protocol).

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Please email us at with any questions. Please inquire about group discounts.


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CBD   Carlsbad, CA   FRD   Frederick, MD   REG   Regional   WEB   Web-Based Training