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CB-101: Cell Culture Basics – Optimizing Lab Techniques (3 Days)

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P/N: A29795

Price: $1260

 

Course Description:

Mammalian cell culture is an integral tool in many workflows of biological study, and this course is designed to provide key tips and best practices for maintaining healthy cell lines and obtaining the most accurate and reproducible results in downstream applications.

Who Should Attend?

This basic-level course is designed for life science professionals or students with little or no direct experience working in a tissue culture laboratory, or those wishing to refresh or expand on past experience.

Prerequisites:

Some background knowledge of biology and high-level familiarity with structure and functions of cells is expected.

Course Content and Objectives:

Lectures include reviews of basic cell culture concepts and terminology, biosafety and usage of key equipment for optimal sterile technique, growth characteristics of typical mammalian continuous cell lines, important considerations for successful cryopreservation, overview of transfection technologies, methods to confirm cell identity and health status, and brief introduction to concepts in more advanced primary and stem cell culture.

Laboratory sessions include cell counting and passaging of both adherent and suspension mammalian cell lines, cryopreservation (freezing, thawing and viability monitoring), lipid transfection and electroporation, and cell health assays.

At the conclusion of this training, participants should be able to:

  • Understand key variables for maintaining healthy cultures of mammalian continuous cell lines and how to optimize conditions for different cell types.
  • Successfully freeze and thaw cell stocks with maximum viability and keep thorough records for reference in publication or troubleshooting.
  • Choose an appropriate transfection method for specific cell types and experimental goals, and understand how to optimize conditions for lipid transfection and electroporation.
  • Describe various methods to evaluate cell health and perform quality control. 
  • Understand some basic differences in culturing of primary or stem cells for potential future experiments.

Daily Schedule:

Tuesday 8:00am - 6:00pm     (Extra time on the first day to maximize the lab experience.)

Wednesday 9:00am - 5:00pm

Thursday 9:00am - 4:30pm

Other Details:

Tuition includes all course materials, laboratory reagents, and consumables.  Light breakfast, snacks and lunch are also provided each day. Travel, accommodation, and other incidental expenses relating to the training are the responsibility of the attendee.

For questions or more information, please contact us at customertraining@thermofisher.com or 800-711-2088.

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CBD   Carlsbad, CA   FRD   Frederick, MD   REG   Regional   WEB   Web-Based Training
Start date End date Start time End time Hours Price Location
October 3, 2017 October 5, 2017 8:00am PT 5:00pm PT $1,260.00