LabCoat Live Courses
MB-101: Molecular Biology Basics for PCR Applications (2 days)
$1750 (with 12 P.A.C.E.® contact hours)
Who Should Attend?
This basic, introductory-level course is designed for people with little or no experience in a molecular biology laboratory or for those wishing to refresh past experience. Attendees will benefit from having a combination of classroom training and hands-on experience in the laboratory, as well as from interacting closely with fellow attendees and application scientists over a 2-day period.
This course covers basic theoretical concepts and laboratory techniques of molecular biology, as they apply to PCR-based applications such as pharmacogenomics, gene expression analysis, genotyping, and DNA sequencing. Lecture topics include nucleic acid structure and function, DNA replication, nucleic acid purification, design and theory of the polymerase chain reaction (PCR), and real-time PCR theory and fundamentals of data analysis. Laboratory exercises include DNA purification and quantification, PCR, analysis of DNA by gel electrophoresis, and real-time PCR assays and analysis.
At the conclusion of this training, participants should be able to:
• Explain and describe basic features of DNA and how it functions in a eukaryotic cell.
• Determine an appropriate nucleic acid preparation technique for different starting sample types.
• Interpret fragment size and quantity in DNA samples by gel electrophoresis and other analysis methods.
• Recognize the difference between PCR and real-time PCR and know when to apply each of them.
• Apply course concepts to design basic experiments using PCR amplification.
The Thermo Fisher Scientific Training Center is an approved provider of continuing education programs in the clinical laboratory sciences by The American Society for Clinical Laboratory Science (ASCLS) Professional Acknowledgment for Continuing Education (P.A.C.E.®) program.
Assessment criteria: class participation and quiz.
Participants successfully passing the quiz (80%) will be awarded 12 contact hours.
Tuition includes all course materials, laboratory reagents, and consumables. Light breakfast, snacks and lunch are also provided. Travel, accommodation, and other incidental expenses relating to the training are the responsibility of the attendee.
Technology required: none from class partipant. During the class, particpant will have access to laptops and laboratory equipments.
For more information about our course schedule, please contact us at email@example.com.
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