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RT-203: Principles of Quantitative PCR and Data Analysis (3 days)

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P/N: 4479754


COVID-19 Update:  We are actively monitoring the situation with COVID-19. Currently the course dates on the calendar are tentative due to travel and visitor restrictions. You may either:

  • Reserve a spot in tentative course date below (no payment will be collected at this time), or
  • Reserve a spot in the January 1 2021 - December 31, 2021 one below to be added to the sign-up list to be contacted for future courses. (No payment will be collected at this time.) Once we are able to hold classes we will contact you to discuss future course dates and locations.

For any courses rescheduled due to travel restrictions we are happy to transfer your registration to a future course date. Please contact with any questions.


Who Should Attend?

This course is designed for any real-time PCR users seeking a better understanding of all aspects of qPCR Experiments. Attendees will benefit from having a combination of theoretical training in a classroom setting and hands-on experience in the laboratory, as well as from interacting closely with fellow attendees and Thermo Fisher Scientific application scientists over a 3-day period.


This class assumes attendees have prior knowledge of basic molecular biology concepts, including theory of PCR design and applications and experience with basic laboratory techniques such as pipetting and working with solutions. This requirement can be fulfilled by attending MB-101: Molecular Biology Basics for PCR Applications, a 2-day class that can be scheduled just prior to RT-203. Please inquire about discounted pricing for combining MB-101 and RT-203.

Course Description:

This interactive course is a 3-day comprehensive real-time PCR course focusing on quantitative and gene expression applications training that covers all aspects of experimental design, execution, and interpretation. The course is designed to teach following: (1) how to properly design various qPCR experiments; (2) how to design assays; (3) how to validate critical experimental parameters; (4) how to properly set up and run reactions; (5) how to obtain and interpret final data; and (6) how to troubleshoot data. Participants will listen to classroom lectures, as well as carry out laboratory exercises geared toward each of these steps. Course specific theoretical topics and exercises include:


  • Basic real-time PCR theory
  • Assay design
  • Experimental development and gene expression validation workflows
  • Software set up and data analysis, data interpretation, and troubleshooting
  • Basic instrument maintenance
  • qPCR applications
  • Troubleshooting


Practical labs include:

  • Quantification using a standard curve
  • Gene expression assay efficiency determination
  • Relative quantification determination for mRNA


Tuition includes course materials, laboratory reagents, consumables, breakfast, and lunch. Travel, accommodation, and other incidental expenses relating to the training are the responsibility of the attendee.

For information about our course schedule, please contact us at

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CBD   Carlsbad, CA   FRD   Frederick, MD   REG   Regional   WEB   Web-Based Training
Start date End date Start time End time Hours Price Location
January 1, 2021 December 31, 2021 n/a n/a $2,725.00