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RT-203: Principles of Quantitative PCR and Data Analysis (3 days)

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P/N: 4479754

Price: $2595

Who Should Attend?

This course is designed for any real-time PCR users seeking a better understanding of all aspects of qPCR Experiments. Attendees will benefit from having a combination of theoretical training in a classroom setting and hands-on experience in the laboratory, as well as from interacting closely with fellow attendees and Thermo Fisher Scientific application scientists over a 3-day period.

Prerequisites:

This class assumes attendees have prior knowledge of basic molecular biology concepts, including theory of PCR design and applications and experience with basic laboratory techniques such as pipetting and working with solutions. This requirement can be fulfilled by attending MB-101: Molecular Biology Basics for PCR Applications, a 2-day class that can be scheduled just prior to RT-203. Please inquire about discounted pricing for combining MB-101 and RT-203.

Course Description:

This interactive course is a 3-day comprehensive real-time PCR course focusing on quantitative and gene expression applications training that covers all aspects of experimental design, execution, and interpretation. The course is designed to teach following: (1) how to properly design various qPCR experiments; (2) how to design assays; (3) how to validate critical experimental parameters; (4) how to properly set up and run reactions; (5) how to obtain and interpret final data; and (6) how to troubleshoot data. Participants will listen to classroom lectures, as well as carry out laboratory exercises geared toward each of these steps. Course specific theoretical topics and exercises include:

Lectures:

  • Basic real-time PCR theory
  • Assay design
  • Experimental development and gene expression validation workflows
  • Software set up and data analysis, data interpretation, and troubleshooting
  • Basic instrument maintenance
  • qPCR applications
  • Troubleshooting

     

Practical labs include:

  • Quantification using a standard curve
  • Gene expression assay efficiency determination
  • Relative quantification determination for mRNA

     

Tuition includes course materials, laboratory reagents, consumables, breakfast, and lunch. Travel, accommodation, and other incidental expenses relating to the training are the responsibility of the attendee.

For information about our course schedule, please contact us at customertraining@thermofisher.com.

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CBD   Carlsbad, CA   FRD   Frederick, MD   REG   Regional   WEB   Web-Based Training
Start date End date Start time End time Hours Price Location
January 23, 2019 January 25, 2019 9:00am ET 5:00pm ET $2,595.00
February 27, 2019 March 1, 2019 9:00am PT 5:00pm PT $2,595.00
April 10, 2019 April 12, 2019 9:00am PT 5:00pm PT $2,595.00